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Red Koi and Glowing Larvae: A Pond Study Day

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Red Koi and Glowing Larvae: A Pond Study Day

Compare how people changed the traits of carp and mosquitoes.

Part 1: The koi club's breeding round

Read the passage and the two histograms, then answer questions 1–5.

Pond Study Day at Alder Creek

1Mr. Ewan Achterberg's eighth grade class spent a pond study day at the Alder Creek Nature Center. Students swept dip nets through the cattails and put drops of pond water under microscopes. Paloma Szabo found water fleas, green strands of algae and a few mosquito larvae wriggling head down. Next to the wild pond is a small display pond full of koi, with orange, red, white and black patches.

2A guide explained that koi are a colorful kind of common carp. Wild carp are mostly a dull olive-bronze. About 200 years ago, farmers in Japan raised carp in ponds as food and noticed a few fish with red or white patches. They kept those fish and bred them, and over many generations the bright patterns of today's koi appeared. A local koi club still breeds koi and gives young fish to the nature center.

3In 2022 the club raised 200 young koi from its usual parent fish. At six months old, each fish was photographed and scored by the share of its skin covered in red patches. For the next round, the club chose 8 parents only from fish that scored 60% or more. In 2024, it raised 200 young from those parents in the same pond, on the same food, and scored them the same way at the same age.

4After lunch, Dr. Nnamdi Valcourt from the county mosquito control lab spoke to the class. The yellow fever mosquito lays its eggs in small pools of water such as flowerpot saucers and old tires. Only females bite, and they can spread viruses such as dengue and Zika. Male mosquitoes do not bite. The county is testing an engineered strain of this mosquito. Its males carry a gene that scientists built from bacterial and viral DNA. Daughters that inherit the gene die as larvae, while sons survive. The strain also carries a marker gene from a coral that makes larvae glow red under a special microscope light.

5To check the strain, the lab set up two cages, each with 50 regular females. Cage A also got 50 regular males, and Cage B got 50 engineered males. The lab raised 400 eggs from each cage in trays of water and counted the larvae and the adults that came out. Paloma's group looked at the larvae under the special light.

2022 young koi (200 fish, from the club's usual parents)
01020304050607080020406080100Skin with red patches (%)Young koi
2024 young koi (200 fish, from parents that scored 60% or more)
01020304050607080020406080100Skin with red patches (%)Young koi
1.
Use the two histograms. Count the fish in the 60–80% and 80–100% bars together.

In 2022, % of the young koi scored 60% or more. In 2024, % of the young koi scored 60% or more.

2.

Use the 80–100% bars. The number of young koi in this bar in 2024 was times the number in 2022.

3.
Use paragraph 3. Why did the club raise both rounds of young koi in the same pond, on the same food, and score them at the same age?
Question 3 options
4.
Which description best explains how the club changed the 2024 histogram?
Question 4 options
5.
Every 2024 parent scored 60% or more, yet 10 of their young scored less than 20%. Which explanation fits the page best?
Question 5 options

Part 2: The mosquito cage test

Use paragraphs 4 and 5 and the table to answer questions 6–9.

Cage Test: Young From 400 Eggs per Cage
CageFathersLarvae countedLarvae that glowAdult femalesAdult males
ARegular males3720178176
BEngineered males3703700174
6.
Use the table. The first blank is a number of mosquitoes. In the second blank, round to the nearest whole percent.

Cage B produced fewer adult females than Cage A. Counting females and males together, Cage B produced about % as many adults as Cage A.

7.
Every larva in Cage B glowed, but none in Cage A did. Which explanation fits paragraphs 4 and 5 best?
Question 7 options
8.
Suppose the county releases many engineered males in a neighborhood. Based on the cage test, what would most likely happen to the number of biting mosquitoes there over the next few generations?
Question 8 options
9.
The lab wants to show that the engineered gene, not something about the cages, caused the missing females. Circle the two results that, taken together, are the best evidence.

Part 3: Comparing the two technologies

Use everything on this page to answer questions 10–12.

10.
Sort each step by the technology it belongs to.
11.
Which statement best compares the two technologies on this page?
Question 11 options
12.
A visitor says, "If the koi club keeps choosing the reddest fish as parents for many more generations, it will end up with koi that glow red under the special microscope light." Make a claim about this idea. Use evidence from the koi histograms and the mosquito cage test, and explain your reasoning about how each technology changes traits.